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验证数据展示

  • IF Staining of HeLa using CL488-68084

    IF Staining of HeLa using CL488-68084

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 C1QBP antibody (CL488-68084, Clone: 1F9B1 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68084

    FC experiment of HeLa using CL488-68084

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human C1QBP (CL488-68084, Clone:1F9B1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated C1QBP Monoclonal antibody

C1QBP Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68084

产品说明书

CloneNo. 1F9B1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68084-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68084 targets C1QBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 C1QBP fusion protein Ag19773 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 1, q subcomponent binding protein
别名 gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32
计算分子量 282 aa, 31 kDa
观测分子量32 kDa
GenBank蛋白编号BC013731
基因名称 C1QBP
Gene ID (NCBI) 708
RRIDAB_2923881
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07021
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1QBP antibody CL488-68084Download protocol
FC protocol for CL Plus 488 C1QBP antibody CL488-68084Download protocol
Standard Protocols
Click here to view our Standard Protocols