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验证数据展示

  • WB analysis using 85719-4-RR (same clone as 85719-4-PBS)

    WB analysis using 85719-4-RR (same clone as 85719-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85719-4-RR (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85719-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85719-4-RR (same clone as 85719-4-PBS)

    IF Staining of HepG2 using 85719-4-RR (same clone as 85719-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (85719-4-RR, Clone: 250093H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85719-4-PBS in a different storage buffer formulation.

C1QA Recombinant monoclonal antibody, PBS Only

C1QA Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85719-4-PBS

产品说明书

CloneNo. 250093H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Complement C1q A chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy C1QA Ready-To-Use IHC Kit. C1QA primary antibody included.

产品信息

85719-4-PBS targets C1QA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg2396

Product name: Recombinant Human C1QA protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 23-245 aa of NM_015991.4

Sequence: EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKVGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVSGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 1, q subcomponent, A chain
别名 Complement C1q A chain
计算分子量26kDa
观测分子量 26-30 kDa
GenBank蛋白编号NM_015991.4
基因名称 C1QA
Gene ID (NCBI) 712
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02745
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

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