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验证数据展示

  • WB analysis using 85719-4-RR

    WB analysis using 85719-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85719-4-RR (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85719-4-RR

    IF Staining of HepG2 using 85719-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (85719-4-RR, Clone: 250093H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C1QA Recombinant antibody

C1QA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85719-4-RR

产品说明书

CloneNo. 250093H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Complement C1q A chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse lung tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C1QA Ready-To-Use IHC Kit. C1QA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85719-4-RR targets C1QA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C1QA fusion protein Eg2396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 1, q subcomponent, A chain
别名 Complement C1q A chain
计算分子量26kDa
观测分子量 26-30 kDa
GenBank蛋白编号NM_015991.4
基因名称 C1QA
Gene ID (NCBI) 712
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02745
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
WB protocol for C1QA antibody 85719-4-RRDownload protocol
IF protocol for C1QA antibody 85719-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols