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  • Featured Product
  • KD/KO Validated

C19orf61 Polyclonal antibody

C19orf61 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24797-1-AP

Print datasheet

Synonyms

C19orf61, F17127_1, Protein smg 9 homolog, Protein SMG9, SMG9

验证数据展示

  • WB analysis of HeLa using 24797-1-AP

    WB analysis of HeLa using 24797-1-AP

    WB result of C19orf61 antibody (24797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf61 transfected HeLa cells.

  • WB analysis of HeLa using 24797-1-AP

    WB analysis of HeLa using 24797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24797-1-AP (C19orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24797-1-AP

    IP experiment of HeLa using 24797-1-AP

    IP result of anti-C19orf61 (IP:24797-1-AP, 3ug; Detection:24797-1-AP 1:500) with HeLa cells lysate 2000ug.

  • IF Staining of HeLa using 24797-1-AP

    IF Staining of HeLa using 24797-1-AP

    Immunofluorescent analysis of HeLa cells using 24797-1-AP (C19orf61 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24797-1-AP is C19orf61 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human
Immunogen C19orf61 fusion protein Ag14965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 61
Synonyms C19orf61, F17127_1, Protein smg 9 homolog, Protein SMG9, SMG9
Calculated Molecular Weight 520 aa, 58 kDa
Observed Molecular Weight 57-65 kDa
GenBank Accession NumberBC008869
Gene Symbol C19orf61
Gene ID (NCBI) 56006
RRIDAB_2879731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0W8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf61 antibody 24797-1-APDownload protocol
IF protocol for C19orf61 antibody 24797-1-APDownload protocol
IP protocol for C19orf61 antibody 24797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols