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C19orf21 Polyclonal antibody

C19orf21 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26338-1-AP

Print datasheet

Synonyms

C19orf21, MISP, Mitotic interactor and substrate of PLK1

验证数据展示

  • WB analysis of MCF-7 using 26338-1-AP

    WB analysis of MCF-7 using 26338-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26338-1-AP

    IF Staining of HepG2 using 26338-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26338-1-AP (C19orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26338-1-AP is C19orf21 Fusion Protein expressed in E. coli.

Tested Applications IF, WB,ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen C19orf21 fusion protein Ag23712 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 21
Synonyms C19orf21, MISP, Mitotic interactor and substrate of PLK1
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC052236
Gene Symbol C19orf21
Gene ID (NCBI) 126353
RRIDAB_2880482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.

实验方案

Product Specific Protocols
WB protocol for C19orf21 antibody 26338-1-APDownload protocol
IF protocol for C19orf21 antibody 26338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma.

Authors - Xinyang Huang
View Article
humanWB

Cell Death Discov

Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.

Authors - Bin Zhou
View Article
mouseWB,IF

J Vet Med Sci

Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice

Authors - Koki Hiura
  • KO Validated
View Article
humanWB,IF,IP

Int J Mol Sci

Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

Authors - Koki Hiura
  • KD Validated
View Article