验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HepG2 cells |
| Positive IF/ICC detected in | HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26338-1-AP targets C19orf21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag23712 Product name: Recombinant human C19orf21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 330-679 aa of BC052236 Sequence: LLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 21 |
| 别名 | MISP, Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC052236 |
| 基因名称 | C19orf21 |
| Gene ID (NCBI) | 126353 |
| RRID | AB_2880482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVT2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| IF protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma. | ||
Cell Death Discov Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. | ||
J Vet Med Sci Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice
| ||
Int J Mol Sci Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway
| ||
Front Pharmacol Protein and metabolic profiles of tyrosine kinase inhibitors co-resistant liver cancer cells | ||
Adv Sci (Weinh) MISP Suppresses Ferroptosis via MST1/2 Kinases to Facilitate YAP Activation in Non-Small Cell Lung Cancer |
