验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HuH-7 cells, U2OS cells, mouse pancreas tissue, rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31161-1-AP targets C18orf24 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | C18orf24 fusion protein Ag34772 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 18 open reading frame 24 |
| 别名 | C18orf24, Ska1 |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC015706 |
| 基因名称 | C18orf24 |
| Gene ID (NCBI) | 220134 |
| RRID | AB_3669875 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96BD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C18orf24(Spindle and kinetochore-associated protein 1, SKA1) is involved in chromosome congression and mitosis. It is upregulated and oncogenic in several human cancers (PMID: 31827398). In the human genome, SKA1, SKA2, and SKA3 constitute the SKA complex; this is necessary to maintain spindle microtubule attachment to kinetochores during mitosis (PMID: 36397719).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C18orf24 antibody 31161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |