验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
24384-1-AP targets NCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20021 Product name: Recombinant human C17orf85 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 16-340 aa of BC148856 Sequence: SNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 17 open reading frame 85 |
| 别名 | C17orf85, ELG, HSA277841, Nuclear cap-binding protein subunit 3, Protein ELG |
| 计算分子量 | 620 aa, 70 kDa |
| 观测分子量 | 71-80 kDa |
| GenBank蛋白编号 | BC148856 |
| 基因名称 | NCBP3/C17orf85 |
| Gene ID (NCBI) | 55421 |
| RRID | AB_2879518 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53F19 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NCBP3 antibody 24384-1-AP | Download protocol |
| WB protocol for NCBP3 antibody 24384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
