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NCBP3 Polyclonal antibody

NCBP3 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24384-1-AP

Print datasheet

Synonyms

C17orf85, ELG, HSA277841, NCBP3, Protein ELG

验证数据展示

  • WB analysis using 24384-1-AP

    WB analysis using 24384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24384-1-AP (NCBP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24384-1-AP

    IF Staining of HeLa using 24384-1-AP

    Immunofluorescent analysis of HeLa cells using 24384-1-AP (NCBP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, PC-3 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24384-1-AP targets NCBP3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NCBP3 fusion protein Ag20021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 17 open reading frame 85
Synonyms C17orf85, ELG, HSA277841, NCBP3, Protein ELG
Calculated Molecular Weight 620 aa, 70 kDa
Observed Molecular Weight 71-80 kDa
GenBank Accession NumberBC148856
Gene Symbol C17orf85
Gene ID (NCBI) 55421
RRIDAB_2879518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53F19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.

实验方案

Product Specific Protocols
WB protocol for NCBP3 antibody 24384-1-APDownload protocol
IF protocol for NCBP3 antibody 24384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article