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验证数据展示

  • WB analysis using 17017-1-AP

    WB analysis using 17017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17017-1-AP (C17orf48 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ADPRM Polyclonal antibody

ADPRM Polyclonal Antibody for WB, ELISA
Cat No. 17017-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

C17orf48, ADPRibase Mn, ADPRibase-Mn, ADP-Ribose/CDP-Alcohol Diphosphatase, Manganese Dependent, CDP choline phosphohydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17017-1-AP targets ADPRM in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ADPRM fusion protein Ag9997 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 17 open reading frame 48
别名 C17orf48, ADPRibase Mn, ADPRibase-Mn, ADP-Ribose/CDP-Alcohol Diphosphatase, Manganese Dependent, CDP choline phosphohydrolase
计算分子量 40 kDa
观测分子量45 kDa
GenBank蛋白编号BC001294
基因名称 C17orf48
Gene ID (NCBI) 56985
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3LIE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADPRM (ADP-ribose/CDP-alcohol diphosphatase, manganese dependent) is a manganese-dependent glycophosphatase primarily responsible for the breakdown of molecules such as ADP-ribose, IDP-ribose, CDP-glycerol, CDP-choline and CDP-ethanolamine in the cell. The protein is predicted to be located in the cytoplasm and possesses 2',3'-cyclic nucleotide 2'-phosphodiesterase activity, manganese ion-binding activity, and pyrophosphatase activity. ADPRM plays an important role in embryonic development, particularly in the formation of the heart and lungs. In addition, ADPRM may be involved in immune cell signaling, acting as a second messenger for ADP-ribose to activate TRPM2, which in turn regulates oxidative/nitrosative stress.

实验方案

Product Specific Protocols
WB protocol for ADPRM antibody 17017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols