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验证数据展示

  • WB analysis using 30529-1-AP (same clone as 30529-1-PBS)

    WB analysis using 30529-1-AP (same clone as 30529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30529-1-AP (C16orf75 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30529-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 30529-1-AP (same clone as 30529-1-PBS)

    IF Staining of U-251 using 30529-1-AP (same clone as 30529-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using C16orf75 antibody (30529-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30529-1-PBS in a different storage buffer formulation.

C16orf75 Polyclonal antibody, PBS Only

C16orf75 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30529-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30529-1-PBS targets C16orf75 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32880

Product name: Recombinant human C16orf75 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-147 aa of BC031016

Sequence: GKYVMVMGVVQACSPEPCLQAVKMTDLSDNPIHESMWELEVEDLHRNIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 75
别名
观测分子量 19 kDa
GenBank蛋白编号BC031016
基因名称 C16orf75
Gene ID (NCBI) 116028
RRIDAB_3086351
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96E14
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C16orf75 is also named as BLM-associated protein of 18 kDa (BLAP18), RMI2, hRMI2 and RecQ-mediated genome instability protein 2. C16orf75 is essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. And RMI2 is essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM (PMID: 18923082, PMID: 18923083, PMID: 27977684).

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