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C16orf75 Polyclonal antibody

C16orf75 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30529-1-AP

Print datasheet

Synonyms

BLAP18, C16orf75, hRMI2, RMI2

验证数据展示

  • WB analysis using 30529-1-AP

    WB analysis using 30529-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30529-1-AP (C16orf75 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, MCF-7 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30529-1-AP targets C16orf75 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen C16orf75 fusion protein Ag32880 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 16 open reading frame 75
Synonyms BLAP18, C16orf75, hRMI2, RMI2
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC031016
Gene Symbol C16orf75
Gene ID (NCBI) 116028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96E14
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C16orf75 is also named as BLM-associated protein of 18 kDa (BLAP18), RMI2, hRMI2 and RecQ-mediated genome instability protein 2. C16orf75 is essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. And RMI2 is essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM (PMID: 18923082, PMID: 18923083, PMID: 27977684).

实验方案

Product Specific Protocols
WB protocol for C16orf75 antibody 30529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols