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验证数据展示

  • WB analysis using 30529-1-AP

    WB analysis using 30529-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30529-1-AP (C16orf75 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 30529-1-AP

    IF Staining of U-251 using 30529-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using C16orf75 antibody (30529-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C16orf75 Polyclonal antibody

C16orf75 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30529-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RMI2, RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, MCF-7 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30529-1-AP targets C16orf75 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C16orf75 fusion protein Ag32880 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 75
别名 RMI2, RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18
观测分子量 19 kDa
GenBank蛋白编号BC031016
基因名称 C16orf75
Gene ID (NCBI) 116028
RRIDAB_3086351
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96E14
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C16orf75 is also named as BLM-associated protein of 18 kDa (BLAP18), RMI2, hRMI2 and RecQ-mediated genome instability protein 2. C16orf75 is essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. And RMI2 is essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM (PMID: 18923082, PMID: 18923083, PMID: 27977684).

实验方案

Product Specific Protocols
WB protocol for C16orf75 antibody 30529-1-APDownload protocol
IF protocol for C16orf75 antibody 30529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols