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验证数据展示

  • WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 23923-1-AP (same clone as 23923-1-PBS)

    IHC staining of mouse lung using 23923-1-AP (same clone as 23923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 23923-1-AP (C14orf126 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 23923-1-AP (same clone as 23923-1-PBS)

    IF Staining of A549 using 23923-1-AP (same clone as 23923-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C14orf126 antibody (23923-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

C14orf126 Polyclonal antibody, PBS Only

C14orf126 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23923-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Animalia-specific tRNA deacylase, ATD, D-aminoacyl-tRNA deacylase 2, DTD2, D-tyrosyl-tRNA(Tyr) deacylase 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23923-1-PBS targets C14orf126 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21053

Product name: Recombinant human C14orf126 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-168 aa of BC010618

Sequence: MAEGSRIPQARALLQQCLHARLQIRPADGDVAAQWVEVQRGLVIYVCFFKGADKELLPKMVNTLLNVKLSETENGKHVSILDLPGNILIIPQATLGGRLKGRNMQYHSNSGKEEGFELYSQFVTLCEKEVAANSKCAEARVVVEHGTYGNRQVLKLDTNGPFTHLIEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 126
别名 Animalia-specific tRNA deacylase, ATD, D-aminoacyl-tRNA deacylase 2, DTD2, D-tyrosyl-tRNA(Tyr) deacylase 2
计算分子量 168 aa, 19 kDa
观测分子量19 kDa
GenBank蛋白编号BC010618
基因名称 C14orf126
Gene ID (NCBI) 112487
RRIDAB_3669426
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96FN9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.

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