验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, mouse cerebellum tissue, HepG2 cells, mouse lung tissue |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23923-1-AP targets C14orf126 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | C14orf126 fusion protein Ag21053 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 14 open reading frame 126 |
别名 | EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD |
计算分子量 | 168 aa, 19 kDa |
观测分子量 | 19 kDa |
GenBank蛋白编号 | BC010618 |
基因名称 | C14orf126 |
Gene ID (NCBI) | 112487 |
RRID | AB_3669426 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen Affinity purified |
UNIPROT ID | Q96FN9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.
实验方案
Product Specific Protocols | |
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WB protocol for C14orf126 antibody 23923-1-AP | Download protocol |
IF protocol for C14orf126 antibody 23923-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |