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验证数据展示

  • WB analysis of SGC-7901 using 24824-1-AP

    WB analysis of SGC-7901 using 24824-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24824-1-AP (Vertnin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 24824-1-AP

    IHC staining of human stomach cancer using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 24824-1-AP

    IHC staining of human urothelial carcinoma using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Vertnin Polyclonal antibody

Vertnin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24824-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C14orf115, VRTN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24824-1-AP targets Vertnin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20678

Product name: Recombinant human C14orf115 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-350 aa of BC053325

Sequence: MTSRNQLVQKVLQELQEAVECEGLEGLIGASLEAKQVLSSFTLPTCREGGPGLQVLEVDSVALSLYPEDAPRNMLPLVCKGEGSLLFEAASMLLWGDAGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSEESPEMTSLPPATLEAIFDADVKASCFPSSFSNVWHLYALASVLQRNIYSIYPMRNLKIRPYFNRVIRPRRCDHVPSTLHIMWAGQPLTSHFFRHQYFAPVVGLEEVEAEGAPGVAPALPALAPLSSPAKTLELLNREPGLSYSHLCERYSVTKSTFYRWRRQSQEHRQKVAARFSAKHFLQDSFHRGGVVPLQQFLQRFPEISRSTYYAW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 115
别名 C14orf115, VRTN
计算分子量 702 aa, 78 kDa
观测分子量 80 kDa
GenBank蛋白编号BC053325
基因名称 Vertnin
Gene ID (NCBI) 55237
RRIDAB_2879745
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8Y1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vertnin, also named as VRTN and C14orf115, belongs to the vertnin family. It is an essential factor for development of the embryo in a wide range of organisms.

实验方案

Product Specific Protocols
IHC protocol for Vertnin antibody 24824-1-APDownload protocol
WB protocol for Vertnin antibody 24824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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