验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25583-1-AP targets C13orf30 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, canine |
| 免疫原 |
CatNo: Ag22353 Product name: Recombinant human C13orf30 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-139 aa of BC093659 Sequence: MGQNWKRQQKLWNVPQLPFIRVPPSIYDTSLLKALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQRTIPRKTSAMTRRCPSVLPVSVVLPRAQSKRRQVLRN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 13 open reading frame 30 |
| 别名 | Protein FAM216B, FAM216B |
| 计算分子量 | 139 aa, 16 kDa |
| GenBank蛋白编号 | BC093659 |
| 基因名称 | C13orf30 |
| Gene ID (NCBI) | 144809 |
| RRID | AB_2880139 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N7L0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C13orf30 antibody 25583-1-AP | Download protocol |
| IHC protocol for C13orf30 antibody 25583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
