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验证数据展示

  • IHC staining of human kidney using 25583-1-AP

    IHC staining of human kidney using 25583-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25583-1-AP (C13orf30 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25583-1-AP

    IHC staining of human kidney using 25583-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25583-1-AP (C13orf30 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MDCK using 25583-1-AP

    IF Staining of MDCK using 25583-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using C13orf30 antibody (25583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C13orf30 Polyclonal antibody

C13orf30 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 25583-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

IHC, IF/ICC, ELISA

别名

Protein FAM216B, FAM216B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25583-1-AP targets C13orf30 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, canine
免疫原 C13orf30 fusion protein Ag22353 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 13 open reading frame 30
别名 Protein FAM216B, FAM216B
计算分子量 139 aa, 16 kDa
GenBank蛋白编号BC093659
基因名称 C13orf30
Gene ID (NCBI) 144809
RRIDAB_2880139
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N7L0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for C13orf30 antibody 25583-1-APDownload protocol
IF protocol for C13orf30 antibody 25583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols