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验证数据展示

  • WB analysis of L02 using 25957-1-AP

    WB analysis of L02 using 25957-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25957-1-AP (C11orf80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 25957-1-AP

    IHC staining of human pancreas using 25957-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 25957-1-AP

    IHC staining of human pancreas using 25957-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 40x lens).

C11orf80 Polyclonal antibody

C11orf80 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25957-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C11orf80

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman pancreas tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25957-1-AP targets C11orf80 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C11orf80 fusion protein Ag22895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 80
别名 C11orf80
计算分子量 522 aa, 58 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC028240
基因名称 C11orf80
Gene ID (NCBI) 79703
RRIDAB_2880309
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6T0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf80 locates at the chromosome 11. It has two isoforms with MW 70-75 kDa.

实验方案

Product Specific Protocols
WB protocol for C11orf80 antibody 25957-1-APDownload protocol
IHC protocol for C11orf80 antibody 25957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols