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C11orf80 Polyclonal antibody

C11orf80 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25957-1-AP

Print datasheet

Synonyms

C11orf80

验证数据展示

  • WB analysis of L02 using 25957-1-AP

    WB analysis of L02 using 25957-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25957-1-AP (C11orf80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 25957-1-AP

    IHC staining of human pancreas using 25957-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 25957-1-AP

    IHC staining of human pancreas using 25957-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman pancreas tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25957-1-AP is C11orf80 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen C11orf80 fusion protein Ag22895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 80
Synonyms C11orf80
Calculated Molecular Weight 522 aa, 58 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC028240
Gene Symbol C11orf80
Gene ID (NCBI) 79703
RRIDAB_2880309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf80 locates at the chromosome 11. It has two isoforms with MW 70-75 kDa.

实验方案

Product Specific Protocols
WB protocol for C11orf80 antibody 25957-1-APDownload protocol
IHC protocol for C11orf80 antibody 25957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols