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验证数据展示

  • IHC staining of human kidney using 24975-1-AP

    IHC staining of human kidney using 24975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A549 using 24975-1-AP

    WB analysis of A549 using 24975-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24975-1-AP

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HEK-293 using 24975-1-AP

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C10orf68 antibody (24975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

C10orf68 Polyclonal antibody

C10orf68 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24975-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IHC, IF/ICC, ELISA

别名

Protein BIOT2, Coiled-coil domain-containing protein 7, CCDC7, BIOT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24975-1-AP targets C10orf68 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, monkey
免疫原

CatNo: Ag21682

Product name: Recombinant human C10orf68 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-348 aa of BC125091

Sequence: MVPASASGEGLRELLLVQEGEVGADMSHAHNEVPNERLVVEHQESLSKTKLQVKKQETSTEQPLTTPDKEPNENLILRHQDSMSKSEMQVKEQRTLKGQRIITHDEESGENPMLKHQDSVSKIQVQLEIQETSEGEGRSIPDKNSMFVHQDSVSKLQMQEKKKITPGRERRNTRIVVPNENVISVHQDSKSKLQMQEKKQINSGVERHKTFPLEIKKKDISLEHLLPEEKVLLSRSESQTKKLQAKVTSRKKTDIESLRGALGRRLLNDEFKTQSKSFPGPDIEQLTDAFGRDILKDEFKTRSKSLPETDERLHSTTERGTINDAIKTQLKRKSYPETVLKHLKGVNG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 68
别名 Protein BIOT2, Coiled-coil domain-containing protein 7, CCDC7, BIOT2
计算分子量 628 aa, 71 kDa
观测分子量 65 kDa
GenBank蛋白编号BC125091
基因名称 C10orf68
Gene ID (NCBI) 79741
RRIDAB_2879828
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96M83
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

chromosome 10 open reading frame 68 (C10orf68) is a 628 amino-acid protein, which may have three isoforms. The function of C10orf68 is still non-known and needs to be further studied.

实验方案

Product Specific Protocols
WB protocol for C10orf68 antibody 24975-1-APDownload protocol
IHC protocol for C10orf68 antibody 24975-1-APDownload protocol
IF protocol for C10orf68 antibody 24975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols