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验证数据展示

  • WB analysis using 31251-1-AP

    WB analysis using 31251-1-AP

    Rat liver tissue was subjected to SDS PAGE, followed by western blot with 31251-1-AP (C-Reactive Protein/CRP antibody) at a dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 31251-1-AP

    IHC staining of rat liver using 31251-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31251-1-AP (C-Reactive Protein/CRP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C-Reactive Protein/CRP Polyclonal antibody

C-Reactive Protein/CRP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31251-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

C-Reactive Protein, CRP, PTX 1, PTX1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31251-1-AP targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原

CatNo: Eg0942

Product name: Recombinant rat C-reactive protein protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-230 aa of NM_017096.3

Sequence: HEDMSKQAFVFPGVSATAYVSLEAESKKPLEAFTVCLYAHADVSRSFSIFSYATKTSFNEILLFWTRGQGFSIAVGGPEILFSASEIPEVPTHICATWESATGIVELWLDGKPRVRKSLQKGYIVGTNASIILGQEQDSYGGGFDANQSLVGDIGDVNMWDFVLSPEQINAVYVGRVFSPNVLNWRALKYETHGDVFIKPQLWPLTDCCES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-reactive protein, pentraxin-related
别名 C-Reactive Protein, CRP, PTX 1, PTX1
计算分子量25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号NM_017096.3
基因名称 Crp
Gene ID (NCBI) 25419
RRIDAB_3669919
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48199
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation. This antibody is specific for rat Crp.

实验方案

Product Specific Protocols
IHC protocol for C-Reactive Protein/CRP antibody 31251-1-APDownload protocol
WB protocol for C-Reactive Protein/CRP antibody 31251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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