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验证数据展示

  • WB analysis of human brain using 19017-1-AP (same clone as 19017-1-PBS)

    WB analysis of human brain using 19017-1-AP (same clone as 19017-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19017-1-AP (same clone as 19017-1-PBS)

    IHC staining of mouse brain using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

Brevican Polyclonal antibody, PBS Only

Brevican Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19017-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19017-1-PBS targets Brevican in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13552

Product name: Recombinant human BCAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-350 aa of BC010571

Sequence: LEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPTYPASLTDVSLALNELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brevican
别名
计算分子量 99 kDa
观测分子量 145 kDa, 72kDa, 80 kDa
GenBank蛋白编号BC010571
基因名称 BCAN
Gene ID (NCBI) 63827
RRIDAB_10643526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GW7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

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