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验证数据展示

  • WB analysis of human brain using 19017-1-AP

    WB analysis of human brain using 19017-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 19017-1-AP

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 19017-1-AP

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse brain using 19017-1-AP

    IHC staining of mouse brain using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Brevican Polyclonal antibody

Brevican Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19017-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BCAN, UNQ2525/PRO6018, CSPG7, Brevican core protein, Brain-enriched hyaluronan-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, COLO 320 cells, SH-SY5Y cells, Y79 cells
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19017-1-AP targets Brevican in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag13552

Product name: Recombinant human BCAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-350 aa of BC010571

Sequence: LEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPTYPASLTDVSLALNELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brevican
别名 BCAN, UNQ2525/PRO6018, CSPG7, Brevican core protein, Brain-enriched hyaluronan-binding protein
计算分子量 99 kDa
观测分子量 145 kDa, 72kDa, 80 kDa
GenBank蛋白编号BC010571
基因名称 BCAN
Gene ID (NCBI) 63827
RRIDAB_10643526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GW7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

实验方案

Product Specific Protocols
WB protocol for Brevican antibody 19017-1-APDownload protocol
IHC protocol for Brevican antibody 19017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
Human

Mol Psychiatry

Molecular profiling of the hippocampus of children with autism spectrum disorder

Authors - Lindsay E Rexrode
View Article