Brefeldin A

ATPase (HCT 116):0.2 μM

在HF4.9 和 HF28RA 细胞中, Brefeldin A（25 ng/mL）完全抑制细胞生长。在HF1A3 细胞中,Brefeldin A (75 ng/mL) 完全抑制细胞生长。

Ethanol:2.8 mg/mL (10 mM),DMSO:14 mg/mL (50 mM)

HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)