验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC detected in | human PBMCs, mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-98598 targets Biotin in IF/ICC, FC applications and shows reactivity with n/a samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | n/a |
| 免疫原 |
Biotin-KLH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Biotin |
| 别名 | Vitamin B7, Vitamin H |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE Biotin antibody PE-98598 | Download protocol |
| IF protocol for PE Biotin antibody PE-98598 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
