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验证数据展示

  • WB analysis of pig skin using 67275-1-Ig

    WB analysis of pig skin using 67275-1-Ig

    pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67275-1-Ig

    IHC staining of human placenta using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human placenta using 67275-1-Ig

    IHC staining of human placenta using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human lung using 67275-1-Ig

    IHC staining of human lung using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human lung using 67275-1-Ig

    IHC staining of human lung using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human lung cancer using 67275-1-Ig

    IHC staining of human lung cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human lung cancer using 67275-1-Ig

    IHC staining of human lung cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human colon cancer using 67275-1-Ig

    IHC staining of human colon cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human colon cancer using 67275-1-Ig

    IHC staining of human colon cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human kidney using 67275-1-Ig

    IHC staining of human kidney using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human kidney using 67275-1-Ig

    IHC staining of human kidney using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human stomach cancer using 67275-1-Ig

    IHC staining of human stomach cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human stomach cancer using 67275-1-Ig

    IHC staining of human stomach cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human liver using 67275-1-Ig

    IHC staining of human liver using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • IHC staining of human liver using 67275-1-Ig

    IHC staining of human liver using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BGN/Biglycan antibody included
  • FC experiment of HepG2 using 67275-1-Ig

    FC experiment of HepG2 using 67275-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Biglycan Monoclonal antibody

Biglycan Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67275-1-Ig

产品说明书

CloneNo. 1E3F2
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, FC (Intra), ELISA

别名

BGN, 1E3F2, PG-S1, PGI, PG S1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig skin tissue, HepG2 cells, pig colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human lung tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BGN/Biglycan Ready-To-Use IHC Kit. BGN/Biglycan primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67275-1-Ig targets Biglycan in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, pig
文献引用反应性human
免疫原

CatNo: Ag8012

Product name: Recombinant human Biglycan protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC004244

Sequence: MWPLWRLVSLLALSQALPFEQRGFWDFTLDDGPFMMNDEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLARVPSGLPDLKLLQVVYLHSNNITKVGVNDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 biglycan
别名 BGN, 1E3F2, PG-S1, PGI, PG S1
计算分子量 42 kDa
观测分子量 48 kDa
GenBank蛋白编号BC004244
基因名称 Biglycan
Gene ID (NCBI) 633
RRIDAB_2882544
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

实验方案

Product Specific Protocols
WB protocol for Biglycan antibody 67275-1-IgDownload protocol
IHC protocol for Biglycan antibody 67275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Biglycan regulated colorectal cancer progress by modulating enteric neuron-derived IL-10 and abundance of Bacteroides thetaiotaomicron

Authors - Yuyu Xu
View Article
humanIHC

Sci Rep

Exploring the mystery of colon cancer from the perspective of molecular subtypes and treatment

Authors - Wenhong Lu
View Article