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验证数据展示

  • WB analysis using 66586-1-Ig

    WB analysis using 66586-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 66586-1-Ig

    IHC staining of human kidney using 66586-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A549 using 66586-1-Ig

    WB analysis of A549 using 66586-1-Ig

    WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.

  • IF Staining of HepG2 using 66586-1-Ig

    IF Staining of HepG2 using 66586-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

Beta Galactosidase Monoclonal antibody

Beta Galactosidase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66586-1-Ig

产品说明书

CloneNo. 4F4F4
引用文献(16)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66586-1-Ig targets Beta Galactosidase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit, sheep
免疫原

CatNo: Ag8069

Product name: Recombinant human Beta galactosidase protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 329-677 aa of BC007493

Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 galactosidase, beta 1
别名 GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP
计算分子量 76 kDa
观测分子量 64-66 kDa, 76-85 kDa
GenBank蛋白编号BC007493
基因名称 GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2881946
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

实验方案

Product Specific Protocols
IF protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
IHC protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
WB protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

J Neuroinflammation

PTP1B in astrocytes drives pathogen-induced neurodegeneration.

Authors - Zhicheng He
View Article
human, mouseWB

EBioMedicine

Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation.

Authors - Yi Sun
View Article
human,mouseWB

EBioMedicine

Exposure to high doses of tyre antioxidant 6PPD causes senescence to induce unexplained miscarriage by suppressing BAZ1B-mediated ubiquitination degradation of P21.

Authors - Waiping Zhang
View Article
sheepIF

Antioxidants (Basel)

Effects of Fisetin Treatment on Cellular Senescence of Various Tissues and Organs of Old Sheep

Authors - Charles A Huard
View Article
mouseWB,IHC

Free Radic Biol Med

MnSOD non-acetylation mimic knock-in mice exhibit dilated cardiomyopathy

Authors - Joseph R Schell
View Article
mouseWB

Aging Cell

L-Tryptophan Produced by Bifidobacterium pseudocatenulatum NCU-08 Delays Aging in SAMP8 Mice by Activating the Sirt1/P53/P21/Rb Signaling Pathway

Authors - Tangchang Xu
View Article
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