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  • Featured Product
  • KD/KO Validated

Beta Galactosidase Monoclonal antibody, PBS Only

Beta Galactosidase Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4F4F4

Cat No : 66586-1-PBS

Print datasheet

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

验证数据展示

  • WB analysis using 66586-1-Ig (same clone as 66586-1-PBS)

    WB analysis using 66586-1-Ig (same clone as 66586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66586-1-Ig (same clone as 66586-1-PBS)

    IF Staining of HepG2 using 66586-1-Ig (same clone as 66586-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

    IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66586-1-Ig (same clone as 66586-1-PBS)

    WB analysis of A549 using 66586-1-Ig (same clone as 66586-1-PBS)

    WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

产品信息

66586-1-PBS targets Beta Galactosidase in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, mouse, rat
Immunogen Beta Galactosidase fusion protein Ag8069 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name galactosidase, beta 1
Synonyms Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 64-66 kDa, 76-85 kDa
GenBank Accession NumberBC007493
Gene Symbol GLB1
Gene ID (NCBI) 2720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16278
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.