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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-17565

Print datasheet

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

验证数据展示

  • IF Staining of MCF-7 using CL594-17565

    IF Staining of MCF-7 using CL594-17565

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 Beta Catenin antibody (CL594-17565) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-17565

    FC experiment of MCF-7 using CL594-17565

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Beta Catenin (CL594-17565) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells, T-47D cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 17565-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17565 targets Beta Catenin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Beta Catenin fusion protein Ag11763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2919845
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
IF protocol for CL594 Beta Catenin antibody CL594-17565Download protocol
Standard Protocols
Click here to view our Standard Protocols