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Beta Catenin Recombinant antibody, PBS Only

Beta Catenin Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, Mouse, Rat, Zebrafish

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2J24

Cat No : 80488-1-PBS

Print datasheet

Synonyms

b cat, B-catenin, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

验证数据展示

  • WB analysis using 80488-1-RR (same clone as 80488-1-PBS)

    WB analysis using 80488-1-RR (same clone as 80488-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80488-1-RR (same clone as 80488-1-PBS)

    IF Staining of MCF-7 using 80488-1-RR (same clone as 80488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

产品信息

80488-1-PBS targets Beta Catenin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Zebrafish samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, Pig, Mouse, Rat, Zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, B-catenin, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight92 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)