• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of MCF-7 using CL488-67580

    IF Staining of MCF-7 using CL488-67580

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Beta Arrestin 1 antibody (CL488-67580, Clone: 1H8E4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67580

    FC experiment of HeLa using CL488-67580

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Arrestin 1 (CL488-67580, Clone:1H8E4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Beta Arrestin 1 Monoclonal antibody

Beta Arrestin 1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67580

产品说明书

CloneNo. 1H8E4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ARRB1, ARR1, ARR 1, ARB2, ARB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARRB1 Ready-To-Use IHC Kit. ARRB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67580-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67580 targets Beta Arrestin 1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Beta Arrestin 1 fusion protein Ag7504 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 arrestin, beta 1
别名 ARRB1, ARR1, ARR 1, ARB2, ARB1
计算分子量 47 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC003636
基因名称 Beta Arrestin 1
Gene ID (NCBI) 408
RRIDAB_3084360
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49407
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of beta Arrestin 1 in saliva has been identified as a candidate circadian biomarker.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta Arrestin 1 antibody CL488-67580Download protocol
FC protocol for CL Plus 488 Beta Arrestin 1 antibody CL488-67580Download protocol
Standard Protocols
Click here to view our Standard Protocols