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验证数据展示

  • FC experiment of human PBMCs using CL594-98165

    FC experiment of human PBMCs using CL594-98165

    1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite®594 Anti-Human Beta-2-Microglobulin Rabbit RecAb (CL594-98165, Clone: 241308D5) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL594-98165

    FC experiment of human PBMCs using CL594-98165

    1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite®594 Anti-Human Beta-2-Microglobulin Rabbit RecAb (CL594-98165, Clone: 241308D5) (red) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite®594 Anti-Human Beta-2-Microglobulin Rabbit Recombinant Antibody

Beta-2-Microglobulin Uni-rAb® Recombinant Antibody for FC
Cat No. CL594-98165

产品说明书

CloneNo. 241308D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

B2M, 241308D5, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98165-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-98165 targets Beta-2-Microglobulin in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2284

Product name: Recombinant Human Beta-2-Microglobulin protein (mFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-mFc

Tag: C-mFc

Domain: 21-119 aa of BC032589

Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, 241308D5, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3
计算分子量 119 aa, 14 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
激发激光Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61769
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
FC protocol for CL594 Beta-2-Microglobulin antibody CL594-98165Download protocol
Standard Protocols
Click here to view our Standard Protocols
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