验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, mouse brain tissue, C2C12 cells, 4T1 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66799-1-Ig targets Bcl2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Bcl2 fusion protein Ag24261 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | B-cell leukemia/lymphoma 2 |
| 别名 | Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | NM-009741 |
| 基因名称 | Bcl2 |
| Gene ID (NCBI) | 12043 |
| RRID | AB_2882142 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Bcl2 antibody 66799-1-Ig | Download protocol |
| IF protocol for Bcl2 antibody 66799-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia | ||
Biomed Pharmacother Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice | ||
Redox Biol Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. | ||
Antioxidants (Basel) Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress | ||
Cell Prolif Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy. | ||
Int J Mol Sci Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses |
