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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60423-1-PBS in a different storage buffer formulation.

  • WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60423-1-PBS in a different storage buffer formulation.

BUB1B Monoclonal antibody, PBS Only

BUB1B Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60423-1-PBS

产品说明书

CloneNo. 3B2D5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, Indirect ELISA

别名

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BUB1B Ready-To-Use IHC Kit. BUB1B primary antibody included.

产品信息

60423-1-PBS targets BUB1B in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 BUB1B fusion protein Ag34182 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 1 homolog beta (yeast)
别名 BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A
计算分子量 1050 aa, 120 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC018739
基因名称 BubR1
Gene ID (NCBI) 701
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60566
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).