验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, Saos-2 cells, U2OS cells, HeLa cells, HEK-293 cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60423-1-Ig targets BUB1B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | BUB1B fusion protein Ag34182 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | budding uninhibited by benzimidazoles 1 homolog beta (yeast) |
| 别名 | BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A |
| 计算分子量 | 1050 aa, 120 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | BC018739 |
| 基因名称 | BubR1 |
| Gene ID (NCBI) | 701 |
| RRID | AB_3670258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BUB1B antibody 60423-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |