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验证数据展示

  • WB analysis of MCF-7 using 16482-1-AP (same clone as 16482-1-PBS)

    WB analysis of MCF-7 using 16482-1-AP (same clone as 16482-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16482-1-AP (same clone as 16482-1-PBS)

    IF Staining of HepG2 using 16482-1-AP (same clone as 16482-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

BTN2A2 Polyclonal antibody, PBS Only

BTN2A2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16482-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BTN2A2 Ready-To-Use IHC Kit. BTN2A2 primary antibody included.

产品信息

16482-1-PBS targets BTN2A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9603

Product name: Recombinant human BTN2A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-523 aa of BC014021

Sequence: VSICCIKKLQREKKILSGEKKVEQEEKEIAQQLQEELRWRRTFLHAADVVLDPDTAHPELFLSEDRRSVRRGPYRQRVPDNPERFDSQPCVLGWESFASGKHYWEVEVENVMVWTVGVCRHSVERKGEVLLIPQNGFWTLEMFGNQYRALSSPERILPLKESLCRVGVFLDYEAGDVSFYNMRDRSHIYTCPRSAFTVPVRPFFRLGSDDSPIFICPALTGASGVMVPEEGLKLHRVGTHQSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 butyrophilin, subfamily 2, member A2
别名
计算分子量 523 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC014021
基因名称 BTN2A2
Gene ID (NCBI) 10385
RRIDAB_2228110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVV5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

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