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验证数据展示

  • WB analysis of MCF-7 using 16482-1-AP

    WB analysis of MCF-7 using 16482-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16482-1-AP

    IF Staining of HepG2 using 16482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

BTN2A2 Polyclonal antibody

BTN2A2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16482-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

BTF2, BT2.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, NIH/3T3 cells, MCF-7 cells, human testis tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BTN2A2 Ready-To-Use IHC Kit. BTN2A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16482-1-AP targets BTN2A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BTN2A2 fusion protein Ag9603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 butyrophilin, subfamily 2, member A2
别名 BTF2, BT2.2
计算分子量 523 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC014021
基因名称 BTN2A2
Gene ID (NCBI) 10385
RRIDAB_2228110
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVV5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

实验方案

Product Specific Protocols
WB protocol for BTN2A2 antibody 16482-1-APDownload protocol
IF protocol for BTN2A2 antibody 16482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols