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验证数据展示

  • WB analysis using 15415-1-AP (same clone as 15415-1-PBS)

    WB analysis using 15415-1-AP (same clone as 15415-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15415-1-AP (same clone as 15415-1-PBS)

    IHC staining of human colon cancer using 15415-1-AP (same clone as 15415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15415-1-AP (BTF3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15415-1-AP (same clone as 15415-1-PBS)

    IF Staining of HeLa using 15415-1-AP (same clone as 15415-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTF3 antibody (15415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

BTF3 Polyclonal antibody, PBS Only

BTF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15415-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15415-1-PBS targets BTF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7701

Product name: Recombinant human BTF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC008062

Sequence: MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic transcription factor 3
别名 OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b
计算分子量 22 kDa
观测分子量 18 kDa, 23 kDa
GenBank蛋白编号BC008062
基因名称 BTF3
Gene ID (NCBI) 689
RRIDAB_3669206
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20290
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Basic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).

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