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验证数据展示

  • WB analysis using 15415-1-AP

    WB analysis using 15415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15415-1-AP

    IHC staining of human colon cancer using 15415-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15415-1-AP (BTF3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15415-1-AP

    IF Staining of HeLa using 15415-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTF3 antibody (15415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BTF3 Polyclonal antibody

BTF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15415-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, HeLa cells, HuH-7 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15415-1-AP targets BTF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BTF3 fusion protein Ag7701 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic transcription factor 3
别名 OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b
计算分子量 22 kDa
观测分子量 18 kDa, 23 kDa
GenBank蛋白编号BC008062
基因名称 BTF3
Gene ID (NCBI) 689
RRIDAB_3669206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20290
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).

实验方案

Product Specific Protocols
WB protocol for BTF3 antibody 15415-1-APDownload protocol
IHC protocol for BTF3 antibody 15415-1-APDownload protocol
IF protocol for BTF3 antibody 15415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols