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验证数据展示

  • WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:10000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

BSEP Monoclonal antibody, PBS Only

BSEP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67512-1-PBS

产品说明书

CloneNo. 3C11D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse , Rat

应用

WB, IHC, IF-P, ELISA

别名

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67512-1-PBS targets BSEP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse , Rat
免疫原

CatNo: Ag29135

Product name: Recombinant human BSEP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 622-750 aa of NM_003742

Sequence: AADTIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFSRGSYQDSLRASIRQRSKSQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAPVRRILKFSAP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family B (MDR/TAP), member 11
别名 ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP
计算分子量 146 kDa
观测分子量 150-160 kDa
GenBank蛋白编号NM_003742
基因名称 BSEP
Gene ID (NCBI) 8647
RRIDAB_2882733
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO95342
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)

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