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CoraLite®594-conjugated BSAP,PAX5 Monoclonal antibody

BSAP,PAX5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H2D6

Cat No : CL594-60349

Print datasheet

Synonyms

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

验证数据展示

  • FC experiment of Ramos using CL594-60349

    FC experiment of Ramos using CL594-60349

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BSAP,PAX5 (CL594-60349, Clone:2H2D6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inRamos cells
For other applications, we recommend the unconjugated version of this antibody, 60349-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60349 targets BSAP,PAX5 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen BSAP,PAX5 fusion protein Ag16023 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name paired box 5
Synonyms BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5
Calculated Molecular Weight 391 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC156927
Gene Symbol PAX5
Gene ID (NCBI) 5079
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02548
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

实验方案

Product Specific Protocols
FC protocol for CL594 BSAP,PAX5 antibody CL594-60349Download protocol
Standard Protocols
Click here to view our Standard Protocols