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验证数据展示

  • WB analysis using 30186-1-AP (same clone as 30186-1-PBS)

    WB analysis using 30186-1-AP (same clone as 30186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 30186-1-AP (same clone as 30186-1-PBS)

    IHC staining of human testis using 30186-1-AP (same clone as 30186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 30186-1-AP (BRPF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

    FC experiment of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

    IF Staining of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRPF1 antibody (30186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

BRPF1 Polyclonal antibody, PBS Only

BRPF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 30186-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

BR140, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30186-1-PBS targets BRPF1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag32828

Product name: Recombinant human BRPF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 741-880 aa of BC053851

Sequence: QAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain and PHD finger containing, 1
别名 BR140, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140
计算分子量 1220 aa, 138 kDa
观测分子量 140 kDa
GenBank蛋白编号BC053851
基因名称 BRPF1
Gene ID (NCBI) 7862
RRIDAB_2935526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55201
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

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