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MPC2 Polyclonal antibody

MPC2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20049-1-AP

Print datasheet

Synonyms

brain protein 44, BRP44, Mitochondrial pyruvate carrier 2, MPC2

验证数据展示

  • IF Staining of HepG2 using 20049-1-AP

    IF Staining of HepG2 using 20049-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20049-1-AP (MPC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293T using 20049-1-AP

    WB analysis of HEK-293T using 20049-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells, mouse brain tissue, LNCaP cells, PC-3 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20049-1-AP targets MPC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain protein 44
Synonyms brain protein 44, BRP44, Mitochondrial pyruvate carrier 2, MPC2
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberNM_015415
Gene Symbol BRP44
Gene ID (NCBI) 25874
RRIDAB_10665371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95563
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRP44 belongs to the UPF0041 (0-44) family. The antibody is specific to BRP44.

实验方案

Product Specific Protocols
WB protocol for MPC2 antibody 20049-1-APDownload protocol
IF protocol for MPC2 antibody 20049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Curr Biol

Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.

Authors - Ridhima Gomkale
View Article
humanWB

Mol Cancer

3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants.

Authors - Eumorphia G Konstantakou
View Article
mouseWB

J Biol Chem

Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production.

Authors - Qian Guo
View Article
humanWB

Hum Mol Genet

Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants

Authors - Stephanie A Zlatic
View Article
humanIF

Life Sci

Mitochondrial pyruvate carrier 2 mediates mitochondrial dysfunction and apoptosis in high glucose-treated podocytes.

Authors - Jun Feng
View Article
ratWB,IF

Brain Res Bull

Downregulation of mitochondrial pyruvate carrier 2 aggravates neuronal injury in the cortex following cerebral ischemia in rat.

Authors - Yue Wang
View Article