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验证数据展示

  • IHC staining of human brain using 16096-1-AP

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRMS1 antibody included
  • IHC staining of human brain using 16096-1-AP

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRMS1 antibody included
  • WB analysis of MCF-7 using 16096-1-AP

    WB analysis of MCF-7 using 16096-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of MCF-7 using 16096-1-AP

    IF Staining of MCF-7 using 16096-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BRMS1 antibody (16096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

BRMS1 Polyclonal antibody

BRMS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16096-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Breast cancer metastasis-suppressor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy BRMS1 Ready-To-Use IHC Kit. BRMS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16096-1-AP targets BRMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human
文献引用反应性human
免疫原 BRMS1 fusion protein Ag8939 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast cancer metastasis suppressor 1
别名 Breast cancer metastasis-suppressor 1
计算分子量 246 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC009834
基因名称 BRMS1
Gene ID (NCBI) 25855
RRIDAB_2878217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCU9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. BRMS1 has since been reported to suppress metastasis in a variety of cancer types such as melanoma, ovarian carcinoma, and non-small lung cancer.

实验方案

Product Specific Protocols
WB protocol for BRMS1 antibody 16096-1-APDownload protocol
IHC protocol for BRMS1 antibody 16096-1-APDownload protocol
IF protocol for BRMS1 antibody 16096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Oncol Rep

BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.

Authors - Jianming Dou
  • KD Validated
View Article
humanWB

Oncol Res

miR-346 Promotes HCC Progression by Suppressing Breast Cancer Metastasis Suppressor 1 Expression

Authors - Zhixian Guo
View Article
humanWB

J Neurooncol

Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM

Authors - Songyun Zhao
View Article