- Featured Product
- KD/KO Validated
MCPH/BRIT1 Polyclonal antibody
MCPH/BRIT1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue |
| Positive IHC detected in | human prostate cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 4 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | MCPH/BRIT1 fusion protein Ag2567 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | microcephalin 1 |
| Synonyms | BRIT1, MCPH1, MCT, Microcephalin, microcephalin 1 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 66 kDa, 100 kDa |
| GenBank Accession Number | BC030702 |
| Gene Symbol | MCPH1 |
| Gene ID (NCBI) | 79648 |
| RRID | AB_2142989 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEM0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| IHC protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.
| ||
Stem Cell Res Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.
| ||
FEBS J Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.
| ||
Genes (Basel) MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.
|
