BRF2 Polyclonal antibody
BRF2 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, DU 145 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12056-1-AP targets BRF2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | BRF2 fusion protein Ag2686 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like |
| Synonyms | B related factor 2, BRF 2, BRF2, BRFU, hBRFU, hTFIIIB50, TFIIIB50 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC010648 |
| Gene Symbol | BRF2 |
| Gene ID (NCBI) | 55290 |
| RRID | AB_2218683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAW0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BRF2 antibody 12056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter |
