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验证数据展示

  • WB analysis using 11702-1-AP (same clone as 11702-1-PBS)

    WB analysis using 11702-1-AP (same clone as 11702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11702-1-AP (BRE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11702-1-AP (same clone as 11702-1-PBS)

    IP experiment of HeLa using 11702-1-AP (same clone as 11702-1-PBS)

    IP result of anti-BRE (IP:11702-1-AP, 4ug; Detection:11702-1-AP 1:1000) with HeLa cells lysate 2320 ug. This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

    IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRE antibody included
  • IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

    IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRE antibody included

BRE Polyclonal antibody, PBS Only

BRE Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11702-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® BRE Ready-To-Use IHC Kit. BRE primary antibody included.

产品信息

11702-1-PBS targets BRE in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2256

Product name: Recombinant human BRE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-383 aa of BC001251

Sequence: PDPSALQNLASWNPSNPECLLLVVKELVQQYHQFQCSRLRESSRLMFEYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDTEATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLSHFGTGVVEYDAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYSPRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brain and reproductive organ-expressed (TNFRSF1A modulator)
别名
计算分子量 383 aa, 44 kDa
观测分子量 40-44 kDa
GenBank蛋白编号BC001251
基因名称 BRE
Gene ID (NCBI) 9577
RRIDAB_2066049
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXR7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

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