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验证数据展示

  • WB analysis using 11702-1-AP

    WB analysis using 11702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11702-1-AP (BRE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11702-1-AP

    IP experiment of HeLa using 11702-1-AP

    IP result of anti-BRE (IP:11702-1-AP, 4ug; Detection:11702-1-AP 1:1000) with HeLa cells lysate 2320 ug.

  • IHC staining of human gliomas using 11702-1-AP

    IHC staining of human gliomas using 11702-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRE antibody included
  • IHC staining of human gliomas using 11702-1-AP

    IHC staining of human gliomas using 11702-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRE antibody included

BRE Polyclonal antibody

BRE Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11702-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® BRE Ready-To-Use IHC Kit. BRE primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

11702-1-AP targets BRE in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag2256

Product name: Recombinant human BRE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-383 aa of BC001251

Sequence: PDPSALQNLASWNPSNPECLLLVVKELVQQYHQFQCSRLRESSRLMFEYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDTEATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLSHFGTGVVEYDAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYSPRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brain and reproductive organ-expressed (TNFRSF1A modulator)
别名 BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2
计算分子量 383 aa, 44 kDa
观测分子量 40-44 kDa
GenBank蛋白编号BC001251
基因名称 BRE
Gene ID (NCBI) 9577
RRIDAB_2066049
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

实验方案

Product Specific Protocols
WB protocol for BRE antibody 11702-1-APDownload protocol
IHC protocol for BRE antibody 11702-1-APDownload protocol
IP protocol for BRE antibody 11702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
View Article
humanWB

Genes Dev

NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.

Authors - Wang Bin B
View Article
humanWB

Int J Biol Sci

PPDPF Promotes the Progression and acts as an Antiapoptotic Protein in Non-Small Cell Lung Cancer.

Authors - Mu Yun
View Article