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验证数据展示

  • IF Staining of HepG2 using CL488-60121

    IF Staining of HepG2 using CL488-60121

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-60121 (BRD8 antibody) at dilution of 1:100.

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  • KD/KO验证

CoraLite® Plus 488-conjugated BRD8 Monoclonal antibody

BRD8 Monoclonal Antibody for IF/ICC
Cat No. CL488-60121

产品说明书

CloneNo. 5B6D6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60121-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60121 targets BRD8 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 BRD8 fusion protein Ag0790 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bromodomain containing 8
别名 BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120
计算分子量 120 kDa
观测分子量 120 kDa
GenBank蛋白编号BC008039
基因名称 BRD8
Gene ID (NCBI) 10902
RRIDAB_2883104
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0E9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BRD8 antibody CL488-60121Download protocol
Standard Protocols
Click here to view our Standard Protocols