BRD8 Recombinant antibody

BRD8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84965-1-RR

产品说明书

CloneNo. 242610F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

SMAP2, SMAP, p120, Bromodomain-containing protein 8, bromodomain containing 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84965-1-RR targets BRD8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 BRD8 fusion protein Ag0790 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bromodomain containing 8
别名 SMAP2, SMAP, p120, Bromodomain-containing protein 8, bromodomain containing 8
计算分子量 120 kDa
观测分子量 120-170 kDa
GenBank蛋白编号BC008039
基因名称 BRD8
Gene ID (NCBI) 10902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0E9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)

实验方案

Product Specific Protocols
WB protocol for BRD8 antibody 84965-1-RRDownload protocol
IF protocol for BRD8 antibody 84965-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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