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验证数据展示

  • WB analysis using 84965-1-RR (same clone as 84965-1-PBS)

    WB analysis using 84965-1-RR (same clone as 84965-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84965-1-RR (BRD8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84965-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84965-1-RR (same clone as 84965-1-PBS)

    IF Staining of HeLa using 84965-1-RR (same clone as 84965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (84965-1-RR, Clone: 242610F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84965-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01679-1

    Cytometric bead array standard curve of MP01679-1

    Cytometric bead array standard curve of MP01679-1, BRD8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84965-2-PBS. Detection antibody: 84965-1-PBS. Standard: Ag0790. Range: 0.156-20 ng/mL.

BRD8 Recombinant antibody, PBS Only (Detector)

BRD8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84965-1-PBS

产品说明书

CloneNo. 242610F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

SMAP2, SMAP, p120, Bromodomain-containing protein 8, bromodomain containing 8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84965-1-PBS targets BRD8 as part of a matched antibody pair:

MP01679-1: 84965-2-PBS capture and 84965-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 BRD8 fusion protein Ag0790 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bromodomain containing 8
别名 SMAP2, SMAP, p120, Bromodomain-containing protein 8, bromodomain containing 8
计算分子量 120 kDa
观测分子量 120-170 kDa
GenBank蛋白编号BC008039
基因名称 BRD8
Gene ID (NCBI) 10902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0E9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)