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验证数据展示

  • WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 28486-1-AP (same clone as 28486-1-PBS)

    IP experiment of A549 using 28486-1-AP (same clone as 28486-1-PBS)

    IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 28486-1-AP (same clone as 28486-1-PBS)

    WB analysis of Jurkat using 28486-1-AP (same clone as 28486-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

    IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRD4 antibody included
  • IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

    IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRD4 antibody included
  • IF Staining of HepG2 using 28486-1-AP (same clone as 28486-1-PBS)

    IF Staining of HepG2 using 28486-1-AP (same clone as 28486-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BRD4 Polyclonal antibody, PBS Only

BRD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 28486-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.

产品信息

28486-1-PBS targets BRD4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain containing 4
别名 BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP
计算分子量 1362 aa, 152 kDa
观测分子量200 kDa
GenBank蛋白编号BC035266
基因名称 BRD4
Gene ID (NCBI) 23476
RRIDAB_2918170
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60885
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. This antibody detects the small and the long isoform of BRD4, the molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).

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