验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A549 cells, Jurkat cells, MCF-7 cells, Ramos cells, MDA-MB-231 cells, T-47D cells, SGC-7901 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28486-1-AP targets BRD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bromodomain containing 4 |
| 别名 | BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP |
| 计算分子量 | 1362 aa, 152 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC035266 |
| 基因名称 | BRD4 |
| Gene ID (NCBI) | 23476 |
| RRID | AB_2918170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60885 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. This antibody detects the small and the long isoform of BRD4, the molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for BRD4 antibody 28486-1-AP | Download protocol |
| IHC protocol for BRD4 antibody 28486-1-AP | Download protocol |
| IP protocol for BRD4 antibody 28486-1-AP | Download protocol |
| WB protocol for BRD4 antibody 28486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics A novel ROR1-targeting antibody-PROTAC conjugate promotes BRD4 degradation for solid tumor treatment | ||
Cell Rep Med An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer | ||
J Control Release The HSP90-dependent bioorthogonal PROTAC prodrug system enables tumor-selective and enhanced protein degradation. | ||
Cell Commun Signal Specific sDMA modifications on the RGG/RG motif of METTL14 regulate its function in AML | ||
Phytomedicine Huangqi-Danshen Decoction Alleviates Cardiac Fibrosis Following Myocardial Infarction by Inhibiting USP22 to Enhance the Ubiquitination of BRD4. | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation |
