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验证数据展示

  • WB analysis using 32452-1-AP (same clone as 32452-1-PBS)

    WB analysis using 32452-1-AP (same clone as 32452-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32452-1-AP (BRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32452-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 32452-1-AP (same clone as 32452-1-PBS)

    IP experiment of Jurkat using 32452-1-AP (same clone as 32452-1-PBS)

    IP result of anti-BRD1/BRL (IP:32452-1-AP, 4ug; Detection:32452-1-AP 1:1000) with Jurkat cells lysate 1920 ug. This data was developed using the same antibody clone with 32452-1-PBS in a different storage buffer formulation.

BRD1/BRL Polyclonal antibody, PBS Only

BRD1/BRL Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 32452-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

BR140-like protein, BRD1, BRL, Bromodomain and PHD finger-containing protein 2, Bromodomain-containing protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32452-1-PBS targets BRD1/BRL in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37530

Product name: Recombinant human BRD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 918-1070 aa of BC047508

Sequence: VLPRLETLLQPRKRSRSTCGDSEVEEESPGKRLDAGLTNGFGGARSEQEPGGGLGRKATPRRRCASESSISSSNSPLCDSSFNAPKCGRGKPALVRRHTLEDRSELISCIENGNYAKAARIAAEVGQSSMWISTDAAASVLEPLKVVWAKCSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain containing 1
别名 BR140-like protein, BRD1, BRL, Bromodomain and PHD finger-containing protein 2, Bromodomain-containing protein 1
计算分子量 120 kDa
观测分子量133 kDa
GenBank蛋白编号BC047508
基因名称 BRD1
Gene ID (NCBI) 23774
RRIDAB_3742614
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95696
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa.

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